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HIF-1α expression and lysyl oxidase (LOX) secretion were increased in RT-R-MDA-MB-231 cells compared with MDA-MB-231 cells. (A,B) HIF-1α expression level (A) and secreted LOX level (B) were detected from cell lysates and medium, respectively, by western blot analysis as described in the Methods. Data represent mean values ± standard error (SEM) of three independent experiments. Significance compared with MDA-MB-231, ** p < 0.01.
