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. 2020 Oct 31;21(21):8186. doi: 10.3390/ijms21218186

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Interaction of MINERVA with Keap1 through its C-terminal region. (a) HEK293 cells were co-transfected with HA-tagged Keap1 and either one of the FLAG-tagged MINERVA^FL, MINERVA^ΔN, or MINERVA^ΔC. HA-Keap1 was immunoprecipitated with anti-HA with protein A-Sepharose and the immunoprecipitates were examined by western blots using respective antibodies. Actin was used as a loading control. Full blot can be found in Figure S7. (b) Surface plasmone resonance (SPR) analysis of MINERVA^FL and Keap1 showing micromolar binding affinity between two proteins. The average and standard deviations of k_a, k_d, and K_D are calculated from values obtained from two separate experiments.