TABLE 1.
Specifications of antibodies used in the present study.
| Antibody (clone) | Region specificity | Antigen | Source | Use and dilution |
|---|---|---|---|---|
| Rabbit polyclonal to APP CT695(CT695) | Human, mouse and rat FL-APP and CTFs | C-terminus 22 amino acid residues of β-APP peptide | Thermoscientific, Waltham, MA, United States | WB 1:1000 |
| Biotinylated mouse monoclonal to human Aβ17–24 (4G8) | hAβ | Amino acids residues 17–24 of hAβ peptide | Biolegend, Dedham, MA, United States | IHC 1:500 |
| Rabbit polyclonal to phosphorylated APP (Thr668) | Human phosphorylated APP at Thr668 | Phospho epitopes matching to residues neighboring Thr668 of human APP695 | Cell signaling, Danvers, MA, United States | WB 1:1000 |
| AT100 monoclonal to phospho tau | Human, mouse and rat phospho tau | Epitopes matching to residues neighboring Thr212, Ser214 phosphorylated sites | Thermoscientific Waltham, MA, United States | IHC 1:500 |
| AT8 monoclonal to phospho tau biotinated | Human, mouse and rat phospho tau | Epitopes matching to residues neighboring Ser 202, Thr 205 phosphorylated sites | Thermoscientific Waltham, MA, United States | IHC 1:500 |
| HT7 monoclonal to total tau biotinated | Human specific | Human tau between residue 159 and 163 | Thermoscientific Waltham, MA, United States | IHC 1:500 |
| PHF-1 monoclonal to phospho tau | Human, mouse and rat phospho tau | Epitopes matching to residues neighboring Ser396 and Ser404phosphorylated sites | Prof. Peter Davies Albert Einstein College of Medicine, Manhasset, NY, United States | WB 1:1000 |
| HT7 monoclonal to total tau | Human specific | Human tau between residue 159 and 163 | Thermoscientific Waltham, MA, United States | WB 1:1000 |
| Mouse monoclonal to β-actin (C4) | β-actin | Bird gizzard actin | Santa Cruz, Dallas,TX, United States | WB: 1:1000 |
| ALZ50 monoclonal to phospho tau | Human, mouse and ratphospho tau | Epitopes matching to residues neighboring Phospho Ser phosphorylated sites | Prof. Peter Davies Albert Einstein College of Medicine,Manhasset, NY, United States | WB 1:1000 |
| CP13 monoclonal to phospho tau | Human, mouse and ratphospho tau | Epitopes matching to residues neighboring Ser 202 phosphorylated sites | Prof. Peter Davies Albert Einstein College of Medicine,Manhasset, NY, United States | WB 1:1000 |
| MC1 monoclonal to phospho tau | Human, mouse and rat phospho tau | Epitopes matching to residues neighboring Ser 312-322 phosphorylated sites | Prof. Peter Davies Albert Einstein College of Medicine,Manhasset, NY, United States | WB 1:1000 |
| AT8 monoclonal to phospho tau | Human, mouse and rat phospho tau | Epitopes matching to residues neighboring Ser 202, Thr 205 phosphorylated sites | Thermoscientific Waltham, MA, United States | WB 1:1000 |
| AT180 monoclonal to phospho tau | Human, mouse and rat phospho tau | Epitopes matching to residues neighboring Thr 231 phosphorylated sites | Thermoscientific Waltham, MA, United States | WB 1:1000 |