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. 2020 Oct 26;25(21):4950. doi: 10.3390/molecules25214950

Figure 3.

Figure 3

Figure 3

The effects of coriolic acid on cancer cell viability and mammosphere-forming efficiency. (A) MDA-MB-231, MCF-7, and HEK-293 cells were cultured with coriolic acid for 1 day. The cytotoxicity of coriolic acid was tested using the MTS assay. (B) Coriolic acid inhibits the colony formation of breast cancer cells incubated and treated with coriolic acid. (C) The effects of coriolic acid on the migration of breast cancer cell lines. Migrations with/without coriolic acid were imaged at 0 and 16 h (scale bar: 100 µm). The percent of inhibition in cell migration was expressed using untreated well at 100%. (D) Coriolic acid suppresses mammosphere-forming capacity. To make mammospheres, 0.5 × 104 MDA-MB-231 cells and 4 × 104 MCF-7 cells per well were plated in 6-well ultralow-attachment 6-well plates. The mammospheres were then cultured with coriolic acid. Representative mammospheres in the photos were obtained by inverted light microscopy (scale bar: 250 µm). The MFE was determined. Representative data were collected. The data from triplicate experiments are represented as the mean ± SD; * p < 0.005, ** p < 0.01.