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. 2020 Oct 29;21(21):8059. doi: 10.3390/ijms21218059

Figure 3.

Figure 3

Effect of 7-HYB on the late osteoblast differentiation. (A–D) Preosteoblasts were cultured in OS with 0.1, 1, 10, and 30 μM of 7-HYB, and mineralized nodule formation was assessed by ARS staining at seven days (A) and 14 days (B). The staining of ARS was detected using a scanner (top) and colorimetric detector (bottom). Mineralization nodules were visualized using a light microscope (C). The intensity of mineralized nodule formation was quantified by eluting ARS stains with DMSO, and measured by the Multiskan GO microplate spectrophotometer (D). Data represent the mean ± S.E.M. of experiments. *, p < 0.05: statistically significant difference when compared to the control. #, p < 0.05: statistically significant difference when compared to OS.