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Effect of 7-HYB on the cytotoxicity of osteoclast lineages. (A) Bone marrow macrophages (BMMs) were treated with 7-HYB (10 and 30 μM) for 24 h. (B,C) BMMs were differentiated into osteoclasts with RANKL (100 ng/mL) in the absence and presence of 7-HYB (10 and 30 μM) for three days (B) and five days (C). Cell viability was measured using the MTT assay. Data represent the mean ± S.E.M. of experiments. ns: no significant difference when compared to the control.
