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. 2020 Oct 28;21(21):8032. doi: 10.3390/ijms21218032

Table 1.

Sequences of customised primers for RT-qPCR.

Gene Forward Primer Sequence Reverse Primer Sequence
NOS3 5′-GTGATGGCGAAGCGAGTGAAG-3′ 5′-CCGAGCCCGAACACACAGAAC-3′
IL6 5′-GGCACTGGCAGAAAACAACC-3′ 5′-GCAAGTCTCCTCATTGAATCC-3′
CXCL8 5′-CAGAGACAGCAGAGCACAC-3′ 5′-AGTTCTTTAGCACTCCTTGGC-3′
VCAM1 5′-CGTCTTGGTCAGCCCTTCCT-3′ 5′-ACATTCATATACTCCCGCATCCTTC-3′
ICAM1 5′-TTGGGCATAGAGACCCCGTT-3′ 5′-GCACATTGCTCAGTTCATACACC-3′
SELE 5′-GCACAGCCTTGTCCAACC-3′ 5′-ACCTCACCAAACCCTTCG-3′
NAI1 5′-CAGACCCACTCAGATGTCAAGAA-3′ 5′-GGGCAGGTATGGAGAGGAAGA-3′
SNAI2 5′-ACTCCGAAGCCAAATGACAA-3′ 5′-CTCTCTCTGTGGGTGTGTGT-3′
TWIST1 5′-GTCCGCAGTCTTACGAGGAG-3′ 5′-GCTTGAGGGTCTGAATCTTGCT-3′
ZEB1 5′-GATGATGAATGCGAGTCAGATGC-3′ 5′-ACAGCAGTGTCTTGTTGTTGT-3′
CDH5 5′-AAGCGTGAGTCGCAAGAATG-3′ 5′-TCTCCAGGTTTTCGCCAGTG-3′
CDH2 5′-GCTTCTGGTGAAATCGCATTA-3′ 5′-AGTCTCTCTTCTGCCTTTGTAG-3′
PECAM1 5′-TGGCGCATGCCTGTAGTA-3′ 5′-TCCGTTTCCTGGGTTCAA-3′