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. 2020 Oct 29;25(21):5031. doi: 10.3390/molecules25215031

Figure 2.

Figure 2

High concentrations of salinosporamide A are cytotoxic to T cells and leads to apoptosis. (A–C) DIC images (A), the expression of AnnexinV (B), or caspase3/7 (C) in Jurkat T cells obtained from the IncuCyte imaging system after incubation with the indicated concentration of salinosporamide A (0–40 nM) for 24 h. (D) The apoptotic population was identified by an AnnexinV/PI apoptosis assay, after incubation with the indicated concentration of salinosporamide A (0–40 nM). The mean value of three experiments ± SEM is presented. * p < 0.05 between control cells.