Skip to main content
. 2020 Oct 29;25(21):5031. doi: 10.3390/molecules25215031

Figure 4.

Figure 4

Treatment with 10 nM salinosporamide A reduces T cell proliferation. (A, B) Jurkat T cells pre-stained with 1 μM CFSE for 30 min were treated with the indicated concentration of salinosporamide A (0–10 nM) for 24. The percentage of CFSE-positive cells were measured by flow cytometry (A), and microscopic fluorescence images and integrated CFSE intensity were determined using an IncuCyte imaging system (B). The black line refers to CFSE-positive cells according to the negative control (-CFSE). (C) The growth rate of Jurkat T cells treated with the indicated concentration of salinosporamide (0–10 nM) for 72 h was determined by counting the cell number every 24 h. The mean value of three experiments ± SEM is presented. * p < 0.05 between control cells.