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. 2020 Oct 29;25(21):5031. doi: 10.3390/molecules25215031

Figure 6.

Figure 6

Treatment with 10 nM salinosporamide A inhibits T-cell activity following TCR-mediated and PMA/A23187 stimulation. (A) Jurkat T cells pre-treated with the indicated concentration of salinosporamide A (0–10 nM) for 1 h were stimulated with anti-CD3 (20 μg/mL)/CD28 (7 μg/mL) antibodies for 6 h. mRNA levels of il2 and ifng were measured by conventional and quantitative PCR. (B) Jurkat T cells pre-treated with the 10 nM salinosporamide A for 1 h were stimulated with anti-CD3 (20 μg/mL)/CD28 (7 μg/mL) antibodies for the indicated time (0–6 h). mRNA levels of il2 and ifng were measured by conventional and quantitative PCR. (C) Mouse T cells pre-treated with the indicated concentration of salinosporamide A (0–10 nM) or 10 nM salinosporamide A for 1 h were stimulated with PMA (100 nM)/A23187 (1 μM) for 6 h or indicated time (0–6 h). After cells were harvested, the mRNA level of il2 was determined by quantitative PCR. The mean value of three experiments ± SEM is presented. * p < 0.05 between control cells.