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(a) Adenosine assay. The complete assay includes adenosine kinase, myokinase, and creatine kinase for the conversion of adenosine to ATP. Adenosine concentration is determined from the difference in luminescence in the absence and presence of adenosine kinase. Enzymes are listed on the left of arrows and substrates listed on the right. (b) Standard curves for AMP and adenosine.
