Table 3.
1H and 13C NMR chemical shifts [ppm] of acyl substituents of O. carboxidovorans OM5 lipid A. Underling means assigning the signal to the specific group.
| Fatty Acid | Group | 1H | 13C |
|---|---|---|---|
| Olefinic protons/carbons (double bond) | -CH2-HC=CH- | 5.350 | 130.08 |
| -CH2-HC=CH- | 2.016 | 27.49 | |
| Ist –(3-OR’)-FA | α1/α2 | 2.620/2.504 | 41 |
| β | 5.145 | 71.29 | |
| γ | 1.588 | 34.4 | |
| R-CONH-Sug | 171.51 | ||
| R-COO- | 174.53 | ||
| ω | 1.59 | 19.8 | |
| IInd –(3-OR”)-FA | α1/α2 | 2.614/2.401 | 40.9 |
| β | 5.262 | 68 | |
| γ | ND | ND | |
| R-CONH-Sug | 169.72 | ||
| R-COO- | ND | ||
| Ist –[(ω-1)-OR]-VLCFA | ω | 1.222 | 19.8 |
| ω-1 | 4.996 | 73.39 | |
| ω-2 | 1.600/1.486 | 36 | |
| ω-3 | 1.41 | 25.5 | |
| ω-4 and next CH2 groups | 1.59 | 29.8 | |
| R-(COO-) from malic acid | 173.3 | ||
| IInd –[(ω-1)-OR]-VLCFA | ω | 1.222 | 19.8 |
| ω-1 | 4.929 | 73.36 | |
| ω-2 | 1.600/1.488 | 36 | |
| ω-3 | 1.474 | 25.5 | |
| ω-4 and next CH2 groups | 1.59 | 29.8 | |
| R-(COO-) from malic acid | 170.84 | ||
| Ist –(3-OH)-FA with unsubstituted OH group | α1/α2 | 2.339/2.252 | 43.9 |
| β | 3.942 | 68.8 | |
| γ | 1.435 | 37.5 | |
| δ | 1.399 | 25.8 | |
| CH2 groups from acyl chain | 1.266 | 29.9 | |
| IInd – (3-OH)-FA with unsubstituted OH group | α1/α2 | 2.307/2.201 | 43.05 |
| β | 3.833 | 68.8 | |
| γ | 1.435 | 37.6 | |
| δ | 1.399 | 25.7 | |
| CH2 groups from acyl chain | 1.266 | 29.9 | |
| [(ω-1)-OH] VLCFA with unsubstituted OH group | ω-1 | 3.756 | 68.4 |
| ω-2 | 1.387/1.478 | 39.34 | |
| ω-3 | 1.28 | 26.05 | |
| ω-4 and next CH2 groups | 1.59 | 29.94 | |
| Ist Malic acid | α1/α2 | 2.860/2.717 | 39.41 |
| β | 4.505 | 67.16 | |
| COO- | 170.84 | ||
| R-COO- | 175.36 | ||
| IInd Malic acid | α1/α2 | 2.860/2.717 | 39.24 |
| β | 4.446 | 67.41 | |
| COO- | 170.58 | ||
| R-COO- | 173.3 |
ND—not detected.