Table 5.
Sequence similarity of putative proteins involved in lipid A modification in Oligotropha carboxidovorans OM5 and Caulobacter crescentus CB15 with enzymes of known functions from the reference strains: Mesorhizobium loti MAFF303099 and Sinorhizobium meliloti 1021.
| Gene Name (Putative Protein Function) |
gnnA (NAD-Dependent Oxidation of GlcN 3-OH of UDP-GlcNAc) |
gnnB (Transamination to Form UDP-GlcNAc3N) |
acpXL (Acyl Carrier Protein) |
fabZXL (3R)-Hydroxyacyl-ACP Dehydratase |
fabF2XL (Beta-Ketoacyl-ACP Synthase) |
fabF1XL (Beta-Ketoacyl-ACP Synthase) |
adh2XL (Zinc-Binding Dehydrogenase) |
lpxXL (msbB) (VLCFA Acyltransferase to C2’ Position of Lipid A) |
|---|---|---|---|---|---|---|---|---|
| Reference sequence of M. loti MAFF303099 and/or S. meliloti 1021 (old description) |
MAFF_RS12135 (mll2825I) |
MAFF_RS30880 (mll7579) |
MAFF_RS05885 (mlr1174) |
SM_RS09960 (SMc04277) 2 |
MAFF_RS05890 (mlr1176) |
MAFF_RS05895 (mlr1177) |
MAFF_RS05900 (mlr1178) |
MAFF_RS05905 (mlr1179) |
|
Oligotropha carboxydovorans OM5 (DSM1227) % Identity 1 |
OCA5_c26370 32.37 |
OCA5_c26360 55.41 |
acpXL 78.49 |
fabA 50.34 |
OCA5_c15570 43.59 |
fabF1 58.12 |
OCA5_c15550 62.24 |
OCA5_c15540 38.36 |
|
Caulobacter crescentus CB15 % Identity 1 |
CC_1225 25.29 |
CC_3611 53.12 |
CC_1677 39.29 |
CCNA_01989 36.59 |
CC_1678 23.76 |
CC_1678 31.54 |
CC_1569 30.41 |
CC_2250 32.26 |
| CC-3719 30 |
||||||||
| Putative function ascribed on BLAST sequence similarity | oxidoreductase domain protein | DegT/DnrJ/EryC1/StrS aminotransferase | acyl carrier protein AcpXL | beta-hydroxyacylACP dehydratase | beta-ketoacyl synthase | 3-oxoacyl-[ACP] synthase 2 | alcohol dehydrogenase | VLCFA acyltransferase to C2’ position of lipid A |
1 % Identity with reference sequence. 2 M. loti MAFF303099 synthetize VLCFAs using housekeeping FabZ and region in M. loti genome encoding enzymes responsible for VLCFAs biosynthesis does not contain fabZXL homolog. Therefore, respective coding sequence SMc04277 from S. meliloti 1021 was used as reference in similarity searches [9].