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The expression of Beclin-1 and LC3B-II in human NCI-H929 cells treated with 1, 2, 4 × IC50 of chloroform fractions of T. vulgaris (TCF) and A. lappa (ACF) for 24 h was detected by western blot analyses. The relative protein expression of Beclin-1 and LC3B-II was normalized to β-actin. Control: NCI-H929 cell without treatment.
