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. Author manuscript; available in PMC: 2020 Nov 13.
Published in final edited form as: Biochem J. 2020 May 15;477(9):1613–1630. doi: 10.1042/BCJ20200197

Table 3.

Binding kinetics of PAM-derived peptides to K2hPg at 25°C

Peptides kon (×106 Ms−1) koff (×10−4 s−1) KD (kinetics)a (nM)
AGL55NS88.2 1.5 ± 0.1 840 ± 24 59 ± 5
KTI55SS1448 3.3 ± 0.3 1540 ± 150 46 ± 2
VEK75AP53 1.9 ± 0.2 2.7 ± 0.2 0.2 ± 0.01
a.

KD values were calculated from koff/kon. Data were collected from triplicate runs, and presented as the mean ± SD.