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. 2020 Oct 24;21(21):7909. doi: 10.3390/ijms21217909

Figure 2.

Figure 2

The classic methods of ubiquitylome in plant. (A), a single enrichment step approach using UBA (Ub-associated) motif [25]. (B), tandem affinity purification (TAP) approach using poly His-tag-UBQ (Ub) motif [26]. (C), two-step affinity tandem Ub-binding entities (TUBE) [33]. (D), Affinity chromatography using Lys-ε-Gly-Gly (K-ε-GG) specific antibody [29]. (E), the Ub COFRADIC (combined fractional diagonal chromatography) pipeline [34].