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. Author manuscript; available in PMC: 2021 Sep 4.
Published in final edited form as: J Mol Biol. 2020 Jul 28;432(19):5411–5429. doi: 10.1016/j.jmb.2020.07.017

Figure 3. Analysis of dimers SS and VV in the presence of different GdmCl concentrations.

Figure 3.

a) Changes in the DS dimer in SS (purple) and VV (black) dimers, and changes in averaged Rh as a function of the denaturant concentration (blue). The native fraction was normalized by excluding No FRET counts. b) Quantification of the dynamic population of the SS (purple) and VV (black) dimers as a function of the denaturant concentration. c) Quantification of rates from DS to intermediate (kDS→I, continuous lines) and vice versa (kI→DS, dashed lines) for SS (purple) and VV (black) dimers. Values were normalized using rates obtained in native conditions to highlight the changes. d) Differences in free energy change of the DS to intermediate transition using native conditions as reference values. Free energy values are calculated as kcal·mol−1.