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. 2020 Oct 30;25(21):5055. doi: 10.3390/molecules25215055

Figure 2.

Figure 2

Apoptosis induced by HME on HCT-116 cells. Cell were treated for 24 h as reported in Methods. Percentages of AnnexinV/PI double stained-HCT-116 cells were determined by a flow cytometer and compared to untreated cells (control). (A) Mean values ± SD of three separate experiments in triplicate. Means with different letters are significantly different (one-way Anova associated with Tukey’s post hoc test) with * p < 0.05, *** p < 0.001. (B) Representative images: BV3, viable cells (AnnexinV−/PI−); BV4, cells in early apoptosis (AnnexinV+/PI−); BV2, cells in tardive apoptosis (AnnexinV+/PI+); BV1, necrotic cells (AnnexinV−/PI+).