Table 1.
The effect of insulin-transferrin-selenium (ITS) or serum supplementation during in vitro culture (IVC) on the developmental competence of oocytes matured under metabolic stress conditions (PA (high palmitic acid) or HCOMBI NEFAs (a combination of pathophysiological, high concentrations of non-esterified fatty acids).
| Maturation | Culture | Oocytes Used | Cleaved Embryos | Two-Cell Stage | Four-Cell Plus | Fragmented Embryos | Day 7 Blastocysts | Day 8 Blastocysts |
|---|---|---|---|---|---|---|---|---|
| BASAL | BSA | 515 | 395 a (76.69%) | 48 a§ (9.32%) | 255 a (49.51%) | 63 (12.23%) | 105 a (20.38%) | 125 a§ (24.27%) |
| PA | BSA | 270 | 176 b (65.18%) | 42 b (15.55%) | 81 b (30.00%) | 35 (12.96%) | 30 b§ (11.11%) | 37 b (13.70%) |
| HCOMBI | BSA | 527 | 377 ab (71.53%) | 63 ab§ (11.95%) | 221 c (41.93%) | 65 (12.33%) | 87 ab§ (16.50%) | 96 ab§ (18.21%) |
| BASAL | ITS | 510 | 406 §$ (79.6%) | 63 (12.35%) | 250 x (49.01%) | 56§ (10.98%) | 124 (24.31%) | 139 (27.25%) |
| PA | ITS | 278 | 200 $ (71.94%) | 43 (15.46%) | 110 y (39.56%) | 30 (10.79%) | 52 (18.7%) | 68 (24.46%) |
| HCOMBI | ITS | 525 | 387 § (73.71%) | 46 (8.76%) | 235 xy (44.76%) | 84 § (16.00%) | 101 (19.23%) | 120 (22.85%) |
| BASAL | Serum | 488 | 348 jk§ (71.31%) | 73 j (14.95%) | 182 j (37.29%) | 55 (11.27%) | 119 j (24.39%) | 136 j (27.86%) |
| PA | Serum | 247 | 156 j§ (63.15%) | 54 k (21.86%) | 53 k (21.45%) | 30 (12.14%) | 30 k (12.14%) | 45 k (18.21%) |
| HCOMBI | Serum | 377 | 275 k (72.94%) | 55 jk (14.58%) | 149 j (39.52%) | 41 (10.87%) | 88 j (23.34%) | 91 jk (24.13%) |
% values refer to proportions from total number of the used oocytes. Values with different superscripts within the same in vitro culture group and column are significantly different at p < 0.05. Values labeled with a, b and c are used within BSA IVC; x, y is used for culture in the presence of ITS; j, k is used within Serum IVC. Values labeled with “§” or “$” tend to be different from each other at p < 0.1 and > 0.05.