Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 24;21(21):7907. doi: 10.3390/ijms21217907

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

CD90 surface expression does not impact adipogenic differentiation. ASCs from 3 donors were subjected to fluorescence-activated cell sorting (FACS) using an anti-CD90 antibody to obtain CD90^high and CD90^low cells. Cells passing through the FACS procedure omitting the anti-CD90 antibody were used as a control (ctrl). (A) FACS gating strategy. SSC-A = side scatter area, SSC-W: side scatter width, FSC-A: forward scatter area. (B) Flow cytometric analysis of CD90 surface expression after sorting procedure. The mean fluorescence intensity (MFI) was normalized to control cells. Data are displayed as mean + SEM. (C–I) CD90^high, CD90^low, and control cells were subjected to adipogenic differentiation. (C) After 14 days, the rate of adipogenic differentiation was determined microscopically. (D–I) The mRNA expression of (D) PPARγ, (E) GLUT4, (F) adiponectin (ADIPOQ), (G) leptin (LEP), (H) UCP1, and (I) PGC1α was determined by RT-qPCR. TF2B expression was used to normalize the data. Data are displayed as mean + SEM. * p < 0.05, **** p < 0.0001, one-way ANOVA with Tukey correction.