Figure 6.

CD90 knockout has no impact on the adipocyte phenotype in human SGBS cells. CD90-deficient (CD90A and CD90B) and control (NC sgRNA) SGBS were subjected to adipogenic differentiation. (A) UCP1 mRNA expression was determined by RT-qPCR. TF2B expression was used to normalize the data. Data are displayed as mean ± SEM of 3 independent experiments. (B) UCP1 protein expression was determined by Western blot. β-actin was used as a loading control. A representative experiment out of 3 independent experiments is shown. (C–H) Cells were analyzed using Seahorse technology to determine the oxygen consumption rates (OCR). (C) Representative trace, (D) basal respiration, (E) ATP production, (F) proton leak respiration, (G) maximal respiration, and (H) extracellular acidification rate were determined as described in Methods. Data are displayed as mean ± SEM of 3 independent experiments. No significant differences between groups were detected by one-way ANOVA with Tukey correction.