Figure 7.

CD90 knockdown has no impact on adipogenesis in human bone marrow stromal cells (BMSCs). Human BMSCs were derived from 3 donors and transfected with CD90-targeting or scrambled (negative control, NC siRNA) siRNAs. (A,B) The down-regulation of CD90 surface expression was assessed by flow cytometry. (B) The mean fluorescence intensity (MFI) was normalized to control cells. (C–F) CD90 and NC siRNA-transfected cells were subjected to adipogenic differentiation. (C) The amount of formed triglycerides was determined. (D–F) The mRNA expression of (D) adiponectin (ADIPOQ), (E) leptin (LEP), and (F) UCP1 was determined by RT-qPCR. HPRT expression was used to normalize the data. Data are expressed as mean ± SEM. ** p < 0.01, Student’s paired t-test.