Table 1.
Comparison of proteins detected large EV (LEV) and/or small EV (SEV) with previous MS/MS studies on the proteome of isolated human seminal plasma EV. Methods used include ultracentrifugation (UC) and size exclusion chromatography (SEC).
| Reference | Isolation Method | Subfractionation | Number of Identified Proteins | Number (%) of Proteins Shared with Our Study |
|---|---|---|---|---|
| Utleg et al. [25] | UC + SEC | No | 139 | 15 (0.96%) |
| Poliakov et al. [31] | UC + sucrose block gradient | No | 440 | 315 (20.22%) |
| Dubois et al. [32] | UC + SEC + sucrose density gradient | Triton-X100 extracted lipid raft associated proteins | 377 | 265 (20.49%) |
| Yang et al. [33] | UC on sucrose cushion | No | 1474 | 949 (60.91%) |
| García-Rodríguez et al. [34] | UC | Normozoospermic versus non-normozoospermic men | 1282 | Protein IDs not available online |
| Our current study | From vasectomized men. UC into sucrose block gradient + upward displacement into velocity sucrose gradient | LEV and SEV separated by velocity sucrose gradient centrifugation | 1558 |