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. 2020 Oct 29;21(21):8089. doi: 10.3390/ijms21218089

Figure 2.

Figure 2

PD-L1-dependent cell spreading on various matrices. Spheroids from cell lines with low (PCI 13), moderate (PCI 8) and high PD-L1 expression (PCI 52) were seeded onto four differently coated surfaces: collagen type I, fibronectin and Matrigel®, a laminin-rich matrix. Poly-L-lysine served as control. Cell spreading was measured over a period of 72 h. The dotted line represents the initial area. (A–H) Spreading of PCI 8 and 52 spheroids after PD-L1 KD. (I) Exemplified images of cell spreading of NT and PD-L1 KD PCI 8 and PCI 52 spheroids on collagen type I. (J–U) Cell spreading of PD-L1 OE spheroids of PCI 13, PCI 8 and PCI 52. (V) Exemplified images of CV and PD-L1 OE PCI 8 and PCI 13 on collagen type I. Scale bar = 1000 µM, black line depicts spreading area measured and calculated with the ImageJ software. The results are expressed as means ± SD, n = 4; endpoints after 72 h were statistically compared by unpaired Student’s t-test.