Figure 4.

PD-L1-dependent gene expression change. Analysis of PD-L1-dependent expression of cell motility associated genes. Extract from the RT² Profiler™ PCR Array Human Cell Motility for genes relevant to cytoskeletal organization. PD-L1 modulation affects various genes associated with Rho-GTPase regulation. Only genes with more than a 2.0-fold change in gene expression were considered to be relevant for evaluation. KD = siRNA knockdown of PD-L1. OE = plasmid overexpression of PD-L1. For qPCR, 25 ng of cDNA was used. The results were expressed as means ± SD; n = 4.