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. 2020 Oct 27;21(21):7986. doi: 10.3390/ijms21217986

Figure 4.

Figure 4

AT1G72910, AT1G72940, and ADR1-LIKE 2 are NLR genes responsible for the severe growth defects of upf1/3 mutants at 16 °C. (a) Sequences of amiRNAs. amiRNA1 is designed to target both AT1G72910 and AT1G72940, whereas amiRNA2 is designed to target ADR1-LIKE 2. Target sites of amiRNA1 and amiRNA2 are indicated by red and blue lines, respectively. Note that reverse complementary sequences of amiRNA are shown. (b) Decrease in expression levels of AT1G72910, AT1G72940, and ADR1-LIKE 2 by amiRNAs in 35S::amiR-NLRs upf1/3 plants. (c) Escape from growth arrest seen in 35S::amiR-NLRs upf1/3 plants at 16 °C. (d) Decrease in PR1 expression levels in 35S::amiR-NLRs upf1/3 plants. (e) Increased levels of PTC+ transcripts of AT2G45670 in 35S::amiR-NLRs upf1/3 plants. Letters indicate significant difference from one-way ANOVA followed by Tukey’s range tests (p < 0.05).