Figure 5.

Overexpression of AT1G72910, AT1G72940, and ADR1-LIKE 2 results in autoimmunity in the WT background. (a) Validation of overexpression of AT1G72910, AT1G72940, and ADR1-LIKE 2 in transgenic lines in T₁ lines at 16 °C. (b) Induction of a growth arrest phenotype by overexpression of AT1G72910, AT1G72940, and ADR1-LIKE 2 in Col-0 plants at 16 °C. Note that the growth arrest phenotype was not seen in the pad4, eds, and npr1 backgrounds, although AT1G72910, AT1G72940, and ADR1-LIKE 2 were overexpressed. (c) Quantification of fresh weight (mg per seedling) and cotyledon area (cm²) of the transgenic seedlings overexpressing AT1G72910, AT1G72940, or ADR2-LIKE 2 at 16 °C in the Col-0, pad4, eds, and npr1 backgrounds. (d–f) PR1 and ICS1 expression in transgenic seedlings overexpressing AT1G72910 (d), AT1G72940 (e) or ADR2-LIKE 2 (f) at 16 °C in the Col-0, pad4, eds, and npr1 backgrounds. All of the experiments were conducted at 16 °C under long day conditions. Letters indicate significant difference from one-way ANOVA followed by Tukey’s range tests (p < 0.05) ***: p ≤ 0.001.