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Effect of Q3G on Wnt/β-catenin and BMP signaling pathways. Analysis of protein expression was carried out by Western blotting of osteo-induced hBM-MSCs at day 10 differentiation. Q3G was treated with initial differentiation induction (3 days) and it was not present in subsequent media changes. β-actin (for whole cell and cytosolic fraction) and lamin B1 (for nuclear fraction) were used as internal loading control.
