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. 2020 Oct 28;21(21):8044. doi: 10.3390/ijms21218044

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of Q3G on adipogenic differentiation of hBM-MSCs. (a) Effect of Q3G on the intracellular lipid accumulation in adipo-induced hBM-MSCs at day 10 of differentiation. Lipid droplets were stained with Oil Red O and the quantification was carried out by measuring absorbance values of retained dye. Lipid accumulation level was given as relative percentage of Oil Red O dye compared to adipo-induced untreated group. Different letters (a–e) indicate statistically significant difference (p < 0.05) among groups. (b) Effect of Q3G on the expression of perilipin-1 and PPARγ in adipo-induced hBM-MSCs at day 10 differentiation analyzed by immunofluorescence staining. DAPI staining was used to highlight the nucleus of viable cells. Scale bar: 50 μm. Effect of Q3G on the expression of adipogenic marker genes (c) and proteins (d). Analysis of gene and protein expression of adipo-induced hBM-MSCs was carried out by RT-PCR and Western blot, respectively, at day 10 of differentiation. GAPDH and β-actin were used as internal loading controls. Q3G was treated with initial differentiation for 3 days.