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Various types of antigens that have been employed to isolate anti-GPCR antibodies. (a) Extracellular region fused proteins. (b) Whole cells expressing GPCRs on their cellular membrane. (c) Membrane factions expressing GPCRs. (d) Purified whole GPCRs. (e) DNA molecules encoding GPCRs. (f) Extracellular region peptides. (g) Virus-like particles (VLPs) displaying GPCRs on the surface. (h) Reconstituted GPCR–lipid–belt protein (GLB) complexes.
