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. 2020 Oct 29;21(21):8072. doi: 10.3390/ijms21218072

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A,B) Fraction of cells exhibiting spread morphology presented as percentages (mean ± standard deviation) in spreading assays using cell culture-derived ECMs and Matrigel. (A) Spreading of control-transfected and siNRAS#1-treated hPheo1 cells on plates coated with ECMs prepared from untreated hPheo1 cells or Matrigel (+), and uncoated plates (-) after 1 h in serum-free conditions. (B) Spreading of untreated hPheo1 cells on ECMs prepared from control-transfected and siNRAS-treated hPheo1 cells (dark-gray and white bars, respectively), and on uncoated wells (light-grey bar) after 1 h in serum-free conditions. All results are from three independent siRNA experiments. If significant one-way ANOVA result, student’s T-test p values were calculated and corrected ad modum Bonferroni. n.s., no significance.