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NT4 binds the plasma membrane of MDA-MB-231 and is internalized within 30 min. (A) After 5 min, part of the NT4 green signal is on the membrane (yellow arrow); (B) after 15 min, the membrane-bound portion of the peptide has decreased; (C) after 30 min, most of the peptide is inside the cell, localized in endosomes (D); (E) anti-HS antibody colocalizes with NT4 on the membrane and in endosomes.
