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Macropinocytosis of NT4. (A) Confocal experiments of colocalization of NT4 (green) and dextran (red); (B) higher magnification of a confocal image of colocalization, yellow arrows indicate colocalization of NT4 and dextran; (C) measurement of colocalization of NT4 and dextran; (D,E) pre-treatment of MDA-MB-231 cells with amiloride impairs NT4 endocytosis (* p = 0.0461).
