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. 2020 Nov 5;21(21):8280. doi: 10.3390/ijms21218280

Figure 2.

Figure 2

EVs from the oviduct are transferred to the oocytes in dogs (left panel) and pigs (right panel). (Left) Canine oEV incorporation in the canine cumulus-oocyte increases over time. (A,B) negative control, (C,D) 24 h, (E,F) 48 h, and (G,H) 72 h after incubation with (PKH67)-labeled canine oEVs. (I) Incorporation of exosomes as measured by fluorescent intensity over time. Bright field images (A,C,E,G), green fluorescent (PKH67) images (B,D,F,H). Length of scale bars was not indicated in the original study. Reprint with permission from [58]. (Right) Images of oocytes from pigs after 20 h of incubation in the presence of (A,C) negative control or (B,D) 0.4 μg/μL of green florescent (PKH67)-labelled porcine oEVs. (A,B) porcine denuded mature oocytes. (C,D) cumulus-oocyte complex. ZP; zona pellucida, PM; plasma membrane, blue; Hoechst staining, green; PKH67-EVs staining. Scale bars; 50 μm. Reprint with permission from [59].