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. Author manuscript; available in PMC: 2020 Nov 13.
Published in final edited form as: Cell Stem Cell. 2020 Mar 5;26(4):579–592.e6. doi: 10.1016/j.stem.2020.02.001

Figure 5. Immune-Microenvironment of NB Tumors Formed in Mouse-Human Chimeras.

Figure 5.

(A–C) Host T cell, CTL, and macrophage infiltration marked by CD3 (A), CD8 (B), and F4/80 (C) in NB tumors of chimeric mice.

(D) No CD3+ T cell infiltrate was observed in a NB of GEMM (Th-MYCN+ALKF1174L).

(E) Representative fields of IHC staining of CHNB and GEMM NB for CD8a, followed by software analysis for quantifications of IHC and immune scores (yellow, tissue area; dark yellow, nuclei staining; red, DAB staining for CD8a; see STAR Methods).

(F and G) Quantifications of IHC experiments for mouse-specific CD3 (F) and CD8a (G) for comparing immune cell infiltration in tumors of GEMM NB (n = 2) and CHNB (n = 6). Data presented as means; error bars represent SD; *** p value < 0.0001, t test.