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. 2020 Nov 5;21(21):8300. doi: 10.3390/ijms21218300

Figure 1.

Figure 1

Effects of HSP90i and HDACi on cell growth and protein expression in A2780 and A2780CisR cells. (A) A 72 h incubation with the HSP90i luminespib and HSP990 showed a slight decrease in cell proliferation for A2780CisR cells. In A2780, this effect was only achieved with HSP990. (B) A 72 h incubation with the HDACi panobinostat or LMK235 slightly reduced the cellular growth of A2780 and A2780CisR cells. (C) A 48 h incubation with luminespib with IC50, three-fold IC50 and five-fold IC50 or time-dependent incubation with three-fold IC50 of luminespib led to a decrease in the expression of the HSP90 client protein AKT and its phosphorylation (pAKT). The uncropped and labeled immunoblots are shown in Figure S1. (D) A 24 h incubation with panobinostat or LMK235 increased the acetylation of α-tubulin in both cell lines. Data shown are mean ± SEM of three independent experiments or one representative immunoblot out of three. Statistical analysis was performed using t-test. Levels of significance: * p ≤ 0.05, *** p ≤ 0.001.