Figure 4.

Caspase 3/7 activation by single treatment and combinations of panobinostat, luminespib, and cisplatin in A2780CisR cells. A2780CisR cells were preincubated with panobinostat and/or luminespib (lumi) for 48h. Cisplatin (23.4 µM, corresponding to the IC₅₀) or buffer control were added for a further incubation period of 24 h. Caspase 3/7 activation was analyzed by ArrayScan XTI. Cisplatin 50 µM (24 h) served as positive control for caspase 3/7 activation, 0.2% DMSO as vehicle control. Data are the mean ± SD. Statistical analysis to compare the caspase 3/7 activation of the two indicated treatments was performed using t-test. Levels of significance: ns p > 0.05, ** p ≤ 0.01.