Figure 5.

Effects of HDACi or HSP90i preincubation prior to cisplatin on apoptosis-related genes. Gene expression data were obtained by PCR. (A,B) A2780 and A2780CisR cells were treated with the indicated inhibitors or their combinations. For combination treatments panobinostat and/or luminespib were administrated 48 h prior to 24 h cisplatin treatment in an IC₅₀. Cell line specific untreated controls were obtained by 72 h incubation with cell culture medium. The concentrations used (A,B) were the same as for cytotoxicity combination studies (Figure S6 and Table 2). One representative agarose gel is shown. Densitometric evaluation can be found in Figure S10B,C.