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. 2020 Nov 5;21(21):8300. doi: 10.3390/ijms21218300

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of HDACi and HSP90i incubation or preincubation prior to cisplatin on protein expression levels of pro-/antiapoptotic proteins. Representative immunoblot analysis of APAF-1, p21, survivin and α-tubulin (as loading control) in A2780 and A2780CisR cells is shown. For combination treatments, panobinostat (pano) and/or luminespib (lumi) were administrated 48 h prior to 24 h cisplatin treatment with respective IC₅₀ concentrations. Cell line specific control was treated with vehicle for 72 h. Densitometric analysis of the Western blots are shown in Figure S11.