Table 4.

Influence of dual combinations with panobinostat or HSP990 on the cytotoxic activity of cisplatin in CaOV3, OVCAR3, and their cisplatin resistant sublines.

Cell Line	Control (Cisplatin Only)	+ 48h Pretreatment
		HSP990		Panobinostat
		Cisplatin IC50 [µM]	SF	Cisplatin IC50 [µM]	SF
CaOV3	1.92	0.79	2.4 ***	1.04	1.8 ***
CaOV3CisR	4.80	3.20	1.5 **	1.38	3.5 ***
OVCAR3	3.94	2.77	1.4 ns	1.50	2.6 **
OVCAR3CisR	37.7	12.6	3.0 *	8.03	4.7 ***

Data shown are IC₅₀ values and shift factors (SF) obtained from three independent experiments each carried out in triplicate. The concentrations used were 10 nM for panobinostat and 10 nM HSP990. SF were calculated as the ratio of IC₅₀ of cisplatin and the IC₅₀ of the corresponding drug combination. pIC₅₀ and SEM are shown in Table S4. Statistical analysis was performed using t-test. Levels of significance: ns p > 0.05, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.