Table 1.

Antibody table

Antibody name	Antibody dilution	Catalog number	Manufacturer/donator
Follistatin	1:100 (in 5% BSA)	#sc‐365003	Santa Cruz Biotechnology
AKT2	1:1000 (in 3% milk)	#3063	Cell Signaling Technology
Phosphorylated (p)‐AKTThr308	1:1000 (in 3% milk)	#9275	Cell Signaling Technology
p‐AKTSer473	1:1000 (in 3% milk)	#9271	Cell Signaling Technology
p70S6K	1:1000 (in 3% milk)	#9202	Cell Signaling Technology
p‐p70S6KThr389	1:1000 (in 3% milk)	#9205	Cell Signaling Technology
TBC1D4	1:1500 (in 5% BSA)	#ab189890	Abcam
p‐TBC1D4Thr642	1:1000 (in 5% BSA)	#D27E6	Cell Signaling Technology
TBC1D1	1:1000 (in 5% BSA)	#5929	Cell Signaling Technology
p‐TBC1D1T596	1:1000 (in 2% milk)	#6927	Cell Signaling Technology
PAK1	1:1000 (in 5% BSA)	#2602	Cell Signaling Technology
p‐PAK1/2Thr423/402	1:1000 (in 5% BSA)	#2601	Cell Signaling Technology
GLUT4	1:1000 (in 3% milk)	#PA1‐1065	Thermo Fisher Scientific
Hexokinase II	1:1000 (in 3% milk)	#2867	Cell Signaling Technology
IRS1	1 μg/mL (in 2% milk)	#06‐248	Upstate Biotechnology
OXPHOS	1:1000 (in 3% milk)	#MS604‐300	Abcam
PDH‐E1α	1 μg/mL (in 2% milk)	CDPG	D.G. Hardie (University of Dundee, Scotland)
p‐PDH‐E1αSer293 (site 1)	1 μg/mL (in 2% milk)		D.G. Hardie (University of Dundee, Scotland)
p‐PDH‐E1αSer300 (site 2)	1 μg/mL (in 2% milk)		D.G. Hardie (University of Dundee, Scotland)
PDK4	0.25 μg/mL (in 2% milk)		D.G. Hardie (University of Dundee, Scotland)
p‐AMPKThr172	1:1000 (in 3% milk)	#2531	Cell Signaling Technology
Puromycin	1:5000 (in 3% milk)	#MABE343	EMD Millipore