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. 2020 Nov 13;13:153. doi: 10.1186/s13041-020-00686-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — The effects of NRG1 on the CoCl₂-induced protein levels of cleaved caspase-3 and Bcl-X_L. a Representative immunofluorescence image of cells after treatment with 100 µM CoCl₂ in the presence or absence of 10 nM NRG1 for 24 h. Cells were fixed and immunostained with anti-cleaved caspase-3 (red) and counterstained with DAPI (blue). Cleaved caspase-3 staining was significantly higher in CoCl₂-only treated cells than in control cells. NRG1 protected against CoCl₂-induced expression of cleaved caspase-3. b The ratio of cleaved caspase-3-positive cells to the total number of cells (n = 6; **P < 0.01 versus the control group; ^#P < 0.05 versus the CoCl₂ alone group). Scale bars, 20 μm. c Protein expression of Bcl-X_L, cleaved caspase-3, caspase-3 and β-actin was analyzed by western blotting. Representative immunoblots showing SH-SY5Y cells treated with 100 µM CoCl₂ and either PBS or NRG1 (5 nM or 10 nM) for 36 h. d Quantitative analysis of the data in a. Treatment with CoCl₂ significantly increased the expression of cleaved caspase-3. NRG1 attenuated the increase in cleaved caspase-3 expression, as shown by the densitometric values (n = 6; **P < 0.01 versus the control group; ^#P < 0.05, ^{# #}P < 0.01 versus the CoCl₂ alone group). e Quantitative analysis of the data in a. Treatment with CoCl₂ significantly decreased the expression of Bcl-X_L in SH-SY5Y cells. NRG1 inhibited the reduction in Bcl-X_L expression, as shown by the densitometric values (n = 6; *P < 0.05 versus the control group; ^#P < 0.05 versus the CoCl₂ alone group). f Protein expression of myc-tag, cleaved caspase-3, caspase-3, and β-actin was analysed after EAAC1-myc transfection. g Representative immunoblots of SH-SY5Y cells treated with 100 µM CoCl₂ and either PBS or NRG1 (5 nM) for 36 h. Quantitative analysis of the data in f (Mock: n = 6; ***P < 0.001 versus the control group; ^##P < 0.01 versus the CoCl₂ alone group: EAAC1-myc: n = 6; ***P < 0.001 versus the control group; ^###P < 0.001 versus the CoCl₂ alone group). EAAC1 transfection reduced CoCl₂-induced cleaved caspase-3 (n = 6; ⁺⁺⁺P < 0.001)