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. 2020 Nov 10;15(5):1140–1153. doi: 10.1016/j.stemcr.2020.10.006

Figure 1.

Figure 1

GSCs Undergo Meiosis In Vitro on a Feeder Layer of Immortalized Sertoli Cells

(A) Schematic overview of the three-step in vitro meiosis culture system. Bars above the timeline represent the period of relatively highly abundant presence of meiotic prophase I, flower-shaped, and meiotic M-phase/post-M-phase cells.

(B) Graphic of the different stages of in vitro germ cell development.

(C) Different stages of the meiotic prophase in vitro, including leptotene, zygotene, and pachytene-like, stained for SYCP3 (green), centromeres (CREST, white), and DNA (DAPI, blue).

(D) In vitro-generated flower-shaped, meiotic M-phase, and spermatid-like cells, stained for SYCP3 (green), centromeres (CREST, red), PNA (green), and DNA (DAPI, white/blue in merged). Regions inside dashed boxes are shown at higher magnification in the following image. Scale bars, 10 μm.