GSCs Undergo Meiosis In Vitro Most Efficiently at 34°C on SK49 Sertoli Cells
(A–D) Quantification of different cell types of in vitro gametogenesis at (A) 34°C on SK49 Sertoli cells, (B) 34°C on TM4 Sertoli cells, (C) 37°C on SK49 Sertoli cells, and (D) 37°C on TM4 Sertoli cells.
(E) Percentage of zygotene cells at day 5 at 34°C on SK49 Sertoli cells, 34°C on TM4 Sertoli cells, 37°C on SK49 Sertoli cells, and 37°C on TM4 Sertoli cells.
(F) Percentage of zygotene, pachytene-like, and meiotic M-phase cells at day 7 at 34°C and 37°C on SK49 Sertoli cells. Data are presented as the mean ± SEM, n = 3. ∗p < 0.05.