Figure 5.

In Vitro-Formed Flower-Shaped Cells Resemble Premature M-Phase Spermatocytes

(A) Unlike bouquet-stage meiotic cells, the clustered centromeres in the flower-shaped cells (CREST, white) do not co-localize with the protein SUN1 (green).

(B) Like flower-shaped cells, okadaic acid (OA)-induced M-phase cells also form stretches of SYCP3 along the chromosome arms; SYCP3 (red in merged), centromeres (CREST, white in merged), and DNA (DAPI, blue in merged).

(C) As in mitotic and meiotic M-phase cells, α-Tubulin (red) in flower-shaped cells forms stretches that end by co-localizing with the centromeres (CREST, white in merged; SYCP3, green).

(D) The flower-shaped cells frequently appear in pairs of recently divided cells containing about half of the chromosomes; SYCP3 (green), centromeres (CREST, red), and DNA (DAPI, white/blue in merged).

(E) Pairs of flower-shaped cells display an uneven distribution of chromosomes (centromeres) between the first (1#) and the second (2#) cell within a pair; ^∗∗p < 0.01. Scale bars, 5 μm.