Figure 6.

Neuronal Differentiation of db/Corr iPSCs Shows the Ability of Gene Editing to Restore the Defects in Regulation of Early Neuronal Markers

(A) Morphological images of cells on day 15 of directed differentiation of Ctrl, db/db, or db/+ iPSCs. Scale bar, 200 μm.

(B) Total protein was isolated from day 15 differentiated neuronal cells. NESTIN antibody was used to detect its abundance in day 15 neuronal cells from Ctrl, db/db, or db/Corr groups. α-Tubulin was used as housekeeping control as shown in representative western blot.

(C and D) (C) Quantification of NESTIN western blot using ImageJ software. (D) NESTIN mean fluorescent intensity measurement of Ctrl, db/db, and db/Corr iPSC differentiated neuronal cells by flow cytometry.

(E) Flow cytometry analyses of NOGGIN+ cells from differentiated neuronal cells of Ctrl, db/db, and db/Corr iPSCs.

n = 3 independent experiments; data are shown as mean ± SD and analyzed by Student's t test (^∗p < 0.05, ^∗∗p < 0.01).