Table 1.
Validation method parameters for quantification of seven steviol glycosides
| Parameters | steviolbioside | Reb B | ST | Reb F | Reb A | Reb M | Reb D |
|---|---|---|---|---|---|---|---|
| LOD (μg/ml) | 7.3 | 3.1 | 1.6 | 2.3 | 2.8 | 3.4 | 3.1 |
| LOQ (μg/ml) | 20.1 | 9.7 | 6.4 | 9.2 | 10.8 | 12.2 | 10.6 |
| Calibration curve | y = 266.95x + 25.022 | y = 219.28x + 1.9539 | y = 222x + 2.7051 | y = 170.87x-2.5236 | y = 166.02x + 6.0046 | y = 160.66x + 8.6147 | y = 166.18x + 11.745 |
| Mean correlation coefficient (R2) | 0.9988 | 0.9989 | 0.9992 | 0.9991 | 0.9991 | 0.9995 | 0.9994 |
| Linear range (μg/ml) | 20.1–597.0 | 19.4–568.1 | 25.6–3610.4 | 33.7–586.0 | 21.7–4820.0 | 14.4–301.0 | 15.5–567.0 |
| Accuracy (%, n = 3) | 87.3 | 108.1 | 96.5 | 104.3 | 97.4 | 85.6 | 103.8 |
| Injection precision (RSD%, n = 6) | 1.97 | 1.94 | 1.53 | 2.03 | 1.72 | 1.58 | 2.34 |
| Stability (RSD%) | 1.51 | 1.73 | 1.21 | 1.93 | 1.05 | 1.46 | 2.01 |
| System suitability (RSD%, n = 6) | 1.92 | 1.85 | 1.74 | 2.13 | 1.59 | 1.95 | 1.87 |