Fig. 6.

Expression of ALK4^L75A-Fc in vivo inhibits growth and metastasis of breast cancer xenografts. a Orthotopic MDA-MB-231 xenograft growth in the presence or absence of doxycycline induction of ALK4^L75A-Fc. Student’s t test, p < 0.05 (across each of the final four measurements). n = 15 per group. b Bright field and fluorescent images of representative lung tissue samples from MDA-MB-231 xenografted mice in the absence (LV-S14-Mock; n = 12) or presence (LV-S14-ALK4^L75A-Fc; n = 8) of ALK4^L75A-Fc expression. c Number of macroscopic fluorescent metastatic foci in the lungs of mice bearing control or ALK4^L75A-Fc-expressing tumor cells. Fisher’s exact test. d Size of primary MDA-MB-231 orthotopic xenografts at 5 weeks post-inoculation in mice injected intraperitoneally with ALK4^L75A-Fc (10 mg/kg; n = 6) or vehicle (n = 4). e Size of MDA-MB-468 orthotopic xenografts at 5 weeks in the presence (n = 6) or absence (n = 4) of ALK4^L75A-Fc expression