Fig. 5.

Assessment of acrosomal status by FITC-PNA labeling of noncapacitated (black and gray bars) and capacitated (hatched and dotted bars) mouse sperm either with (grey and dotted bars) or without (black and hatched bars) previous incubation with calcium ionophore A23187. Sperm were counted for intact, partial or complete acrosome reaction. The data were expressed as mean ± S.E. Different letters represent statistical differences (p < 0.01) between treatments within each category (intact, partial or complete acrosome reaction). The graph represents the average of 5 biological replicates.