Fig. 3.

IAA-94 inhibits the cyclosporin A-mediated increase in calcium retention capacity (CRC) of cardiac mitochondria. Extramitochondrial calcium was labeled with calcium green 5 N as described previously and the uptake of the calcium was measured in the presence and absence of both IAA-94 (30 μmol L⁻¹) and cyclosporin A (CsA, 1 nmol L⁻¹). (A) In this representative figure, CsA increased the Ca²⁺ pulses required to induce mPTP opening to 21 pulses from 14 pulses (absence of CsA) of 5 μmol L⁻¹ CaCl₂. IAA-94 treatment inhibited the CsA mediated delayed onset of mPTP opening. (B), bar graphs representing average pulses of CaCl₂ required for mPTP opening in both the presence and absence of CsA and IAA-94 in n = 4. Statistical significance values were determined by one way ANOVA test, n.s. is not significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)